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Spero®

Infrared Microscopy Will Never Be the Same.


It’s finally here. A breakthrough approach to infrared microscopy that opens a whole new world of research possibilities. Spero®, from Daylight Solutions, is the world’s first laser-based infrared microscopy platform, representing a true breakthrough in high performance spectral imaging. Spectral imaging allows a user to visualize the chemical content across a sample, and Spero provides this data with higher spatial resolution, larger fields of view, and dramatically faster than existing instruments. And for the first time, chemical imaging can be done in real-time with Spero’s innovative Live Mode.
  • Unparalleled Visibility
Ultra-bright, tunable laser source offers high-resolution with a wide field of view and a novel chemical imaging technique that has not been previously available.
  • Instantaneous Results in Live Mode
Produces hyperspectral data cubes in minutes and also supports live discrete frequency imaging, eliminating standard workflow steps to acquire data.
  • Small Resource Footprint
Not only is the microscope small enough to sit on a desktop,
it’s also easy to use and features an uncooled focal plane array.
 

Spero wins CLEO/LFW Innovation Award


Using infrared chemical imaging to study brain tumors in 3D: an interview Dr Cyril Petibois. To read the whole interview, click here.
      

         
  • Designed specifically for mid-infrared spectral imaging analysis of biomedical and materials research samples
  • High-brightness tunable laser source
  • Spectral coverage across the entire mid-infrared “fingerprint region”
  • Rapid acquisition of full spectrum hyperspectral datacubes
  • 2 infrared objectives and 1 visible objective
 
  • Transmission and reflection modes
  • Large format uncooled focal plane array
  • Real-time discrete frequency IR imaging at video frame rates
  • Fully automated stage
  • Instrument control and data display via Ethernet to PC
  • Small instrument footprint

Product Brochure

Abstract

Spectral Imaging Article

Press Release

Technical Papers

Sreedhar et al. "Infrared spectroscopic imaging detects chemical modifications in liver fibrosis due to diabetes and disease." Biomedical Optics Express 7 6 (2016)
Pilling et al. "High-throughput quantum cascade laser (QCL) spectral histopathology: a practical approach towards clinical translation" - Faraday Discussion, 187 (2016)
Hughes et al. "Introducing Discrete Frequency Infrared Technology for High-Throughput Biofluid Screening", Scientific Reports, 6 20173 (2016)
Bassan et al. “Large scale infrared imaging of tissue micro arrays (TMAs) using a tunable Quantum Cascade Laser (QCL) based microscope. Analyst 139 (2014)
Clemmens et al. “Quantum cascade laser-based mid-infrared spectrochemical imaging of tissues and biofluids.” Spectroscopy Europe. Vol. 26 No. 4 (2014)

Applications Notes and White Papers

Daylight Solutions Logo AN101602 Large‐area infrared chemical imaging of colorectal tissue using a Spero microscope
AN201501 Real-Time Chemical Imaging of Microfluidic System
White Paper: Get more from mass spec imaging using Spero technology


This material is based upon work supported by the National Science Foundation under Grant Number 1230424.